Beschreibung
Im Projekt BioDivSoil wurde untersucht, wie sich die Bodenbiodiversität in der Agrarlandschaft unter verschiedenen Nutzungs- und Bewirtschaftungsformen unterschied und wie sie zuverlässig erfasst und bewertet werden konnte. Dafür wurden an 45 Standorten sowohl morphologische Bestimmungsmethoden als auch molekularbiologische Verfahren wie eDNA-Analysen und DNA-Metabarcoding eingesetzt. Im Fokus standen endogäische Gruppen wie Collembola, Oribatida und Lumbricidae sowie epigäische Prädatoren wie Carabidae und Araneae. Die Ergebnisse zeigten deutliche Unterschiede zwischen Feldrainen, Grünland und intensiv genutzten Ackerflächen und unterstrichen die besondere Bedeutung strukturreicher Landschaftselemente für die Biodiversität in Agrarräumen. Gleichzeitig machte das Projekt sichtbar, dass unterschiedliche Methoden komplementäre Einblicke in Bodenlebensgemeinschaften liefern. BioDivSoil schuf damit eine wichtige Grundlage für die Entwicklung wissenschaftlich fundierter Indikatoren und für eine nachhaltigere landwirtschaftliche Bodennutzung.
Informationen
Fördermittelgeber: Bundesamt für Naturschutz
Mitarbeiter: Lukas Schröer, Lucas Stratemann, Martina Roß-Nickoll
Laufzeit: 2021 – 2024
Homepage: –
Publikationen
2025
Stratemann L; Schröer L; Bach A; Toschki A; Oellers J; Roß-Nickoll M
BioDivSoil – Bodenbiodiversität im Fokus: Diversitätsmuster und Methodenevaluation zur Erfassung und Bewertung der Bodenbiodiversität in der Agrarlandschaft Forschungsbericht
Deutschland / Bundesamt für Naturschutz, BfN-Schriften 746, 2025.
@techreport{Stratemann2025,
title = {BioDivSoil – Bodenbiodiversität im Fokus: Diversitätsmuster und Methodenevaluation zur Erfassung und Bewertung der Bodenbiodiversität in der Agrarlandschaft},
author = {Lucas Stratemann and Lukas Schröer and Alexander Bach and Andreas Toschki and Johanna Oellers and Martina Roß-Nickoll},
editor = {Bundesamt für Naturschutz},
doi = {10.19217/skr746},
year = {2025},
date = {2025-04-01},
urldate = {2025-04-01},
issue = {BfN-Schriften 746},
institution = {Deutschland / Bundesamt für Naturschutz, BfN-Schriften 746,},
abstract = {The identification of biodiversity patterns in agricultural landscapes is of crucial importance for the assessment of ecological status and the development of sustainable management strategies. As part of the BioDivSoil project, soil biodiversity under different forms of land use was investigated using established and molecular biological methods. The aim was to identify indicators for the promotion and evaluation of soil biocenoses.
The soil fauna was recorded at a total of 45 sites and identified to species level using morpho-logical and molecular biological approaches. Collembola, Oribatida and Lumbricidae were an-alyzed as representatives of the endogean communities and Carabidae and Araneae as repre-sentatives of the epigean predators. At the molecular biological level, eDNA analyses of soil samples and DNA metabarcoding of soil trap contents were used.
The data collected in the first year of the study showed clear differences between different forms of land use. Tipping points were identified, which manifested themselves in the disap-pearance of specialized species and the dominance of generalist species on intensively used sites, i.e. intensive grassland and arable land. This pattern was identified for both endogean and epigean animal groups. While Collembola and Oribatida showed a strong decline in the number of species and individuals, this was not the case for Carabidae and Araneae. The mo-lecular-biologically determined bycatches from pitfall traps also showed differences between the land-use types in terms of species composition. Endogean biocenoses identified by eDNA analyses could only be distinguished between field margins and intensive sites.
Both the DNA-based methods and the Carabidae and Araneae showed significantly higher numbers of species in field margins than on arable land, which indicates the enormous im-portance of structural diversity in the agricultural landscape. No significant differences were found between arable land and intensive grassland. The Lumbricidae hardly differed between the three forms of land use.
In the second year of research, organically and conventionally farmed arable land and young fallow fields were compared in terms of their biodiversity. In this part of the project, Carabidae and Araneae were morphologically determined and eDNA analyses from soil samples and DNA metabarcoding of bycatches were carried out. No significant differences were found between organically and conventionally farmed arable land. However, the conventionally farmed ara-ble fields showed a significantly lower number of species and functional richness compared to the fallow fields. The eDNA analysis showed a significantly higher number of species for endo-gean groups on organic plots compared to fallow fields. These results indicate that epigean communities recover faster and respond more quickly to changes in environmental factors than endogean communities.
The BioDivSoil project provides important insights into the assessment of biodiversity in the agricultural landscape and at the same time highlights the need for research and action for sustainable soil management in agriculture.},
keywords = {},
pubstate = {published},
tppubtype = {techreport}
}
The identification of biodiversity patterns in agricultural landscapes is of crucial importance for the assessment of ecological status and the development of sustainable management strategies. As part of the BioDivSoil project, soil biodiversity under different forms of land use was investigated using established and molecular biological methods. The aim was to identify indicators for the promotion and evaluation of soil biocenoses.
The soil fauna was recorded at a total of 45 sites and identified to species level using morpho-logical and molecular biological approaches. Collembola, Oribatida and Lumbricidae were an-alyzed as representatives of the endogean communities and Carabidae and Araneae as repre-sentatives of the epigean predators. At the molecular biological level, eDNA analyses of soil samples and DNA metabarcoding of soil trap contents were used.
The data collected in the first year of the study showed clear differences between different forms of land use. Tipping points were identified, which manifested themselves in the disap-pearance of specialized species and the dominance of generalist species on intensively used sites, i.e. intensive grassland and arable land. This pattern was identified for both endogean and epigean animal groups. While Collembola and Oribatida showed a strong decline in the number of species and individuals, this was not the case for Carabidae and Araneae. The mo-lecular-biologically determined bycatches from pitfall traps also showed differences between the land-use types in terms of species composition. Endogean biocenoses identified by eDNA analyses could only be distinguished between field margins and intensive sites.
Both the DNA-based methods and the Carabidae and Araneae showed significantly higher numbers of species in field margins than on arable land, which indicates the enormous im-portance of structural diversity in the agricultural landscape. No significant differences were found between arable land and intensive grassland. The Lumbricidae hardly differed between the three forms of land use.
In the second year of research, organically and conventionally farmed arable land and young fallow fields were compared in terms of their biodiversity. In this part of the project, Carabidae and Araneae were morphologically determined and eDNA analyses from soil samples and DNA metabarcoding of bycatches were carried out. No significant differences were found between organically and conventionally farmed arable land. However, the conventionally farmed ara-ble fields showed a significantly lower number of species and functional richness compared to the fallow fields. The eDNA analysis showed a significantly higher number of species for endo-gean groups on organic plots compared to fallow fields. These results indicate that epigean communities recover faster and respond more quickly to changes in environmental factors than endogean communities.
The BioDivSoil project provides important insights into the assessment of biodiversity in the agricultural landscape and at the same time highlights the need for research and action for sustainable soil management in agriculture.
The soil fauna was recorded at a total of 45 sites and identified to species level using morpho-logical and molecular biological approaches. Collembola, Oribatida and Lumbricidae were an-alyzed as representatives of the endogean communities and Carabidae and Araneae as repre-sentatives of the epigean predators. At the molecular biological level, eDNA analyses of soil samples and DNA metabarcoding of soil trap contents were used.
The data collected in the first year of the study showed clear differences between different forms of land use. Tipping points were identified, which manifested themselves in the disap-pearance of specialized species and the dominance of generalist species on intensively used sites, i.e. intensive grassland and arable land. This pattern was identified for both endogean and epigean animal groups. While Collembola and Oribatida showed a strong decline in the number of species and individuals, this was not the case for Carabidae and Araneae. The mo-lecular-biologically determined bycatches from pitfall traps also showed differences between the land-use types in terms of species composition. Endogean biocenoses identified by eDNA analyses could only be distinguished between field margins and intensive sites.
Both the DNA-based methods and the Carabidae and Araneae showed significantly higher numbers of species in field margins than on arable land, which indicates the enormous im-portance of structural diversity in the agricultural landscape. No significant differences were found between arable land and intensive grassland. The Lumbricidae hardly differed between the three forms of land use.
In the second year of research, organically and conventionally farmed arable land and young fallow fields were compared in terms of their biodiversity. In this part of the project, Carabidae and Araneae were morphologically determined and eDNA analyses from soil samples and DNA metabarcoding of bycatches were carried out. No significant differences were found between organically and conventionally farmed arable land. However, the conventionally farmed ara-ble fields showed a significantly lower number of species and functional richness compared to the fallow fields. The eDNA analysis showed a significantly higher number of species for endo-gean groups on organic plots compared to fallow fields. These results indicate that epigean communities recover faster and respond more quickly to changes in environmental factors than endogean communities.
The BioDivSoil project provides important insights into the assessment of biodiversity in the agricultural landscape and at the same time highlights the need for research and action for sustainable soil management in agriculture.
2024
Schröer L; Bach A; Oellers J; Nabel M; Kirse A; Stratemann L; Toschki A; Roß-Nickoll M
In: Natur und Landschaft, Bd. 99, Ausg. 09/10, S. 470–479, 2024.
@article{Schröer2024,
title = {Hürden und Chancen der Integration DNA-basierter Methoden für ein Bodenbiodiversitätsmonitoring in Agrarlebensräumen},
author = {Lukas Schröer and Alexander Bach and Johanna Oellers and Moritz Nabel and Ameli Kirse and Lucas Stratemann and Andreas Toschki and Martina Roß-Nickoll},
doi = {10.19217/NuL2024-09-06},
year = {2024},
date = {2024-10-01},
urldate = {2024-10-01},
journal = {Natur und Landschaft},
volume = {99},
issue = {09/10},
pages = {470--479},
abstract = {Trotz steigender Anerkennung der Bedeutung von Bodenorganismen und deren Diversität bestehen auch heute noch große Wissenslücken hinsichtlich der im und auf dem Boden lebenden Organismen. Auf dieser Grundlage entstand die Idee für das BioDivSoil-Projekt, in dem in einem einheitlichen Studiendesign auf drei verschiedenen Standorttypen (Acker, Feldrain, Grünland) Vergleiche zwischen der morphologischen Bestimmung von Organismen (Laufkäfer - Carabidae, Spinnen - Araneae, Springschwänze - Collembola, Hornmilben - Oribatida, Regenwürmer - Lumbricidae) und verschiedenen molekularbiologischen Methoden zur Artbestimmung angestellt werden. So soll die Eignung der molekularbiologischen Methoden als Werkzeug in einem ökologischen Monitoring terrestrischer Lebensräume überprüft werden. Erste Vergleiche der verschiedenen Methoden hinsichtlich der gefundenen Artenzahlen der Gruppen Lumbriciden, Oribatiden und Collembolen zeigen, dass die molekularbiologischen Methoden durchaus Potenzial zur Anwendung innerhalb eines ökologischen Monitorings aufweisen. Dennoch existieren noch große Einschränkungen aufgrund lückenhafter Gendatenbanken, bisher nicht standardisierter Verfahren und der Frage nach der Validierbarkeit der Ergebnisse. Diese Hindernisse müssen auf dem Weg zu einer einheitlichen Anwendung der Methoden im Rahmen eines ökologischen Monitorings von Böden zwingend beseitigt werden.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Trotz steigender Anerkennung der Bedeutung von Bodenorganismen und deren Diversität bestehen auch heute noch große Wissenslücken hinsichtlich der im und auf dem Boden lebenden Organismen. Auf dieser Grundlage entstand die Idee für das BioDivSoil-Projekt, in dem in einem einheitlichen Studiendesign auf drei verschiedenen Standorttypen (Acker, Feldrain, Grünland) Vergleiche zwischen der morphologischen Bestimmung von Organismen (Laufkäfer - Carabidae, Spinnen - Araneae, Springschwänze - Collembola, Hornmilben - Oribatida, Regenwürmer - Lumbricidae) und verschiedenen molekularbiologischen Methoden zur Artbestimmung angestellt werden. So soll die Eignung der molekularbiologischen Methoden als Werkzeug in einem ökologischen Monitoring terrestrischer Lebensräume überprüft werden. Erste Vergleiche der verschiedenen Methoden hinsichtlich der gefundenen Artenzahlen der Gruppen Lumbriciden, Oribatiden und Collembolen zeigen, dass die molekularbiologischen Methoden durchaus Potenzial zur Anwendung innerhalb eines ökologischen Monitorings aufweisen. Dennoch existieren noch große Einschränkungen aufgrund lückenhafter Gendatenbanken, bisher nicht standardisierter Verfahren und der Frage nach der Validierbarkeit der Ergebnisse. Diese Hindernisse müssen auf dem Weg zu einer einheitlichen Anwendung der Methoden im Rahmen eines ökologischen Monitorings von Böden zwingend beseitigt werden.